|CDC Study Debunked
By Gail Kansky Konstance K. Knox, Ph.D., the president and director of Wisconsin Viral Research, severely criticized a recent study that claimed HHV6 (either strain as well as HHV7) had no ties to ME/CFIDS. The Centers for Disease Control and Prevention (CDC) tested patients and healthy controls (Infect Di J, August 2000) using serum, lymphocytes and 3 polymerase chain reaction tests. "These are good people doing good work but not using the correct methodology," explained Dr. Knox. Essentially, the CDC testing investigated:
FIBROBLASTS IN VIALS FOR 48 HOURS
FIX AND STAIN FOR IE ANTIGENS
These are perfect ways to NOT finding the right answer!
The serology testing is a method of finding antibodies in the serum but results dont give results that would be beneficial. In fact, using this particular method would show that 95% of the population positive!
The PCR (polymerase chain reaction) can detect viral DNA but gives no evidence of active infections and HHV6 is an active infection! This method is known for high false negatives because there are many inhibitors found in each sample. The CDC tested both patients and controls using these inaccurate ways of detecting active HHV6 infection. Dr. Knox and Dr. Donald Carrigan have spent 12 years studying HHV6 and rely on none of the tests used by the CDC. "The CDC should be thinking about how A is transmitted," declared Dr. Knox. Dr. William Reeves is not known by her, but she does know 2 of the other authors of the study and one, Dr. Pellet, has had most of his laboratory workers pulled from him. He is now working with a skeleton staff.
Since the government has said they will no longer look for a cause (they feel theyve ruled out everything!), Dr. Knox listed what the CDC has not done and how they have damaged progress.
The CDC is very negative
The CDC cant do good replicative studies (i.e. Dr. Elaine DeFreitas retroviral work and how the HHV6 replication study.
Their latest paper said, "They say no association (of HHV6) so, they think, since they didnt find it, it doesnt exist! In fact, they found more DNA in the controls than the patients!"
Since the CDC used technologies that would not find HHV6, Dr. Knox felt "the CDC doesnt know any better...HHV6 is difficult to find. Its not found in peripheral blood lymphocytes and thats what they were looking at. The total leukocytes must be studied. They used serology. I dont know why. Theres no way to separate normals from those who have active infection. They didnt do controls for inhibitors." In summary, Reeves, et al used methods that cant distinguish between active and latent infections yet some of the authors are top notch people but they do work that is not applicable in this type of work. This is very destructive to HHV6 research. We will address this...their data is incomplete."
"There have been 32 studies of patients and normal controls for HHV6. None of the studies using serology or DNA show 6A but most who used culturing testing found active 6A."
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